Hotspot

trnC-GCA-petN

LSC · 30866-31637 · 772 bp

Back to Ampelocera

Pi 0.0233

MarkerSeek score 69.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 30
pi: 0.0233
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	806-854 bp	1.0	85.1
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.9	58.0	858-906 bp	1.0	84.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	800-848 bp	1.0	83.7
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	57.9	57.8	852-900 bp	1.0	82.9
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	824-872 bp	1.0	82.8