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psbK-psbI

LSC · 8763-9264 · 502 bp

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Pi 0.0241

MarkerSeek score 70.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 20
pi: 0.0241
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	AGGATTACGACCCGGATCAT	45.0	50.0	58.1	58.0	543-633 bp	1.0	86.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	AGGATTACGACCCGGATCAT	50.0	50.0	57.9	58.0	538-628 bp	1.0	86.2
psbK-psbI_p3	GCCAAATTACCCGAGGCTTA	AGGATTACGACCCGGATCAT	50.0	50.0	58.0	58.0	625-715 bp	1.0	85.9
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	AGGATTACGACCCGGATCAT	45.0	50.0	58.3	58.0	535-625 bp	1.0	84.2
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	AGGATTACGACCCGGATCAT	45.0	50.0	58.3	58.0	536-626 bp	1.0	84.2