Hotspot

rpl33-rps18

LSC · 68002-68272 · 271 bp

Back to Ampelocalamus

Pi 0.031

MarkerSeek score 64.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 18
pi: 0.031
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAGCTAAGTCAGGCCTCAG	TGACTGGGCTTGAAATTGCT	55.0	45.0	58.0	58.0	1056-1099 bp	1.0	86.8
rpl33-rps18_p2	TCGCAAGCATACGACTCATG	CGAAAAGTTTGCTTGGATTTACG	50.0	39.1	58.4	57.5	346-418 bp	0.846154	50.9
rpl33-rps18_p3	TGTCGTTATTGTCGCAAGCA	CGAAAAGTTTGCTTGGATTTACG	45.0	39.1	58.5	57.5	357-429 bp	0.846154	50.3
rpl33-rps18_p4	TTGTCGTTATTGTCGCAAGC	CGAAAAGTTTGCTTGGATTTACG	45.0	39.1	57.4	57.5	358-430 bp	0.846154	49.6
rpl33-rps18_p5	CGCAAGCATACGACTCATGA	CGAAAAGTTTGCTTGGATTTACG	50.0	39.1	58.4	57.5	386-417 bp	0.769231	46.0