Hotspot

rbcL-psaI

LSC · 58441-59742 · 1302 bp

Back to Ampelocalamus

Pi 0.00881

MarkerSeek score 55.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1302
variable_sites: 43
pi: 0.00881
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1619-1683 bp	1.0	85.6
rbcL-psaI_p2	GTTATACCGGTGGCTTCAGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1630-1694 bp	1.0	85.3
rbcL-psaI_p3	CGCCGGTACAGTAGTAGGTA	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1769-1833 bp	1.0	85.0
rbcL-psaI_p4	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	2152-2221 bp	1.0	84.9
rbcL-psaI_p5	CTTAACTGGGGGATTCACCG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1984-2007 bp	0.923077	78.3