Hotspot

rps14-psaB

LSC · 39194-39349 · 156 bp

Back to Ampelocalamus

Pi 0.0178

MarkerSeek score 65.10

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 11
pi: 0.0178
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CTCAAGGGGGAAACTTTGCT	TGGATTAGCCCACTTTTCCG	50.0	50.0	58.0	57.9	343-355 bp	1.0	84.0
rps14-psaB_p2	CTCAAGGGGGAAACTTTGCT	GGATTAGCCCACTTTTCCGT	50.0	50.0	58.0	57.9	342-354 bp	1.0	84.0
rps14-psaB_p3	CTCAAGGGGGAAACTTTGCT	GCAGCTTTCTTGATTGCCTC	50.0	50.0	58.0	57.7	303-315 bp	1.0	83.4
rps14-psaB_p4	CTCAAGGGGGAAACTTTGCT	GCTCTTTCCATTGTGCAAGC	50.0	50.0	58.0	58.3	372-384 bp	1.0	82.3
rps14-psaB_p5	CTCAAGGGGGAAACTTTGCT	TGGCTCTTTCCATTGTGCAA	50.0	45.0	58.0	58.3	374-386 bp	1.0	82.2