Hotspot

trnD-GUC-psbM

LSC · 17565-18561 · 997 bp

Back to Ampelocalamus

Pi 0.00771

MarkerSeek score 68.80

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 42
pi: 0.00771
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCA	50.0	40.0	58.5	54.5	1071-1142 bp	1.0	53.8
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TGCGAGAATATTGACTTCCA	55.0	40.0	58.7	54.5	1103-1174 bp	1.0	52.2
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	ATGCGAGAATATTGACTTCCA	50.0	38.1	58.5	55.1	1072-1143 bp	1.0	50.7
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCAT	50.0	38.1	58.5	55.1	1071-1142 bp	1.0	50.7
trnD-GUC-psbM_p5	GTTCAATTGGTCAGAGCACC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.0	54.5	1072-1143 bp	1.0	49.6