primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	20	20	45.0	50.0	58.0	58.2	0.0	5.0	0.209227	8491	9471	1105-1151	1.000000	82.4
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	8491	9471	1092-1138	1.000000	81.8
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	20	20	50.0	50.0	57.6	58.2	0.9	5.0	0.580969	8491	9471	1091-1137	1.000000	79.4
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	20	20	45.0	55.0	58.0	58.6	0.0	0.0	0.605692	8491	9471	1084-1130	1.000000	79.3
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.6	58.2	0.9	0.0	0.656018	8491	9471	1078-1124	1.000000	78.9
trnfM-CAU-trnT-GGU_p1	trnfM-CAU-trnT-GGU	1	CCTCGTGAGCTACCAAACTG	CCATGGCATTACTCTACCACC	20	21	55.0	52.4	58.3	58.1	0.0	0.0	1.434011	13536	16313	2911-2987	1.000000	74.2
trnfM-CAU-trnT-GGU_p2	trnfM-CAU-trnT-GGU	2	CCTCGTGAGCTACCAAACTG	GACTTACGTCTTACCATGGCA	20	21	55.0	47.6	58.3	57.8	0.0	13.0	1.526273	13536	16313	2924-3000	1.000000	73.5
trnfM-CAU-trnT-GGU_p3	trnfM-CAU-trnT-GGU	3	CCTCAAGGTTATGAGCCTCG	CCATGGCATTACTCTACCACC	20	21	55.0	52.4	57.8	58.1	0.0	0.0	1.380020	13536	16313	2926-2972	0.923077	71.5
trnfM-CAU-trnT-GGU_p4	trnfM-CAU-trnT-GGU	4	CCTCAAGGTTATGAGCCTCG	GACTTACGTCTTACCATGGCA	20	21	55.0	47.6	57.8	57.8	0.0	13.0	1.472281	13536	16313	2939-2985	0.923077	70.7
trnfM-CAU-trnT-GGU_p5	trnfM-CAU-trnT-GGU	5	CCTCAAGGTTATGAGCCTCG	TGACTTACGTCTTACCATGGC	20	21	55.0	47.6	57.8	57.8	0.0	13.0	1.472281	13536	16313	2940-2986	0.923077	70.7
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCA	20	20	50.0	40.0	58.5	54.5	5.4	0.0	3.996878	17565	18561	1071-1142	1.000000	53.8
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	TAGGTATGCCATACACCCCG	TGCGAGAATATTGACTTCCA	20	20	55.0	40.0	58.7	54.5	16.3	0.0	4.175481	17565	18561	1103-1174	1.000000	52.2
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	TTCAATTGGTCAGAGCACCG	ATGCGAGAATATTGACTTCCA	20	21	50.0	38.1	58.5	55.1	5.4	0.0	4.379605	17565	18561	1072-1143	1.000000	50.7
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	TTCAATTGGTCAGAGCACCG	TGCGAGAATATTGACTTCCAT	20	21	50.0	38.1	58.5	55.1	5.4	0.0	4.379605	17565	18561	1071-1142	1.000000	50.7
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	GTTCAATTGGTCAGAGCACC	TGCGAGAATATTGACTTCCA	20	20	50.0	40.0	57.0	54.5	5.4	0.0	4.517032	17565	18561	1072-1143	1.000000	49.6
rpoC2-rps2_p1	rpoC2-rps2	1	ATGAGACATCAGAACCCCCA	ACTCCCGCCTCTATCATCTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.110979	31752	32057	379-422	1.000000	86.8
rpoC2-rps2_p2	rpoC2-rps2	2	TGAGACATCAGAACCCCCAT	ACTCCCGCCTCTATCATCTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.110979	31752	32057	378-421	1.000000	86.8
rpoC2-rps2_p3	rpoC2-rps2	3	ATGAGACATCAGAACCCCCA	TGAACTCCCGCCTCTATCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.116955	31752	32057	382-425	1.000000	86.7
rpoC2-rps2_p4	rpoC2-rps2	4	TGAGACATCAGAACCCCCAT	ATGAACTCCCGCCTCTATCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.116955	31752	32057	382-425	1.000000	86.7
rpoC2-rps2_p5	rpoC2-rps2	5	TGAGACATCAGAACCCCCAT	TGAACTCCCGCCTCTATCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.116955	31752	32057	381-424	1.000000	86.7
rps14-psaB_p1	rps14-psaB	1	CTCAAGGGGGAAACTTTGCT	TGGATTAGCCCACTTTTCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.123859	39194	39349	343-355	1.000000	84.0
rps14-psaB_p2	rps14-psaB	2	CTCAAGGGGGAAACTTTGCT	GGATTAGCCCACTTTTCCGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.125353	39194	39349	342-354	1.000000	84.0
rps14-psaB_p3	rps14-psaB	3	CTCAAGGGGGAAACTTTGCT	GCAGCTTTCTTGATTGCCTC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.275996	39194	39349	303-315	1.000000	83.4
rps14-psaB_p4	rps14-psaB	4	CTCAAGGGGGAAACTTTGCT	GCTCTTTCCATTGTGCAAGC	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.294633	39194	39349	372-384	1.000000	82.3
rps14-psaB_p5	rps14-psaB	5	CTCAAGGGGGAAACTTTGCT	TGGCTCTTTCCATTGTGCAA	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.298894	39194	39349	374-386	1.000000	82.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	48397	49212	908-922	1.000000	86.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	48397	49212	900-914	1.000000	86.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	48397	49212	878-892	1.000000	85.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	48397	49212	898-912	1.000000	84.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	48397	49212	907-921	1.000000	84.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.540775	52526	53281	889-1027	1.000000	79.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	57.5	58.3	0.0	22.8	0.770156	52526	53281	890-1028	1.000000	78.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGGTCCTTCACTAATCGGGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.8	57.9	0.0	0.0	0.875460	52526	53281	892-1030	1.000000	77.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	57.4	57.9	0.0	0.0	0.680025	52526	53281	893-898	0.923077	73.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGTCCTTCACTAATCGGG	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	57.4	58.3	0.0	22.8	0.909407	52526	53281	894-899	0.923077	71.4
rbcL-psaI_p1	rbcL-psaI	1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.040121	58441	59742	1619-1683	1.000000	85.6
rbcL-psaI_p2	rbcL-psaI	2	GTTATACCGGTGGCTTCAGG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	19.9	26.9	0.077538	58441	59742	1630-1694	1.000000	85.3
rbcL-psaI_p3	rbcL-psaI	3	CGCCGGTACAGTAGTAGGTA	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	1.9	26.9	0.073444	58441	59742	1769-1833	1.000000	85.0
rbcL-psaI_p4	rbcL-psaI	4	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.071710	58441	59742	2152-2221	1.000000	84.9
rbcL-psaI_p5	rbcL-psaI	5	CTTAACTGGGGGATTCACCG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.065390	58441	59742	1984-2007	0.923077	78.3
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	57.9	57.8	8.4	0.0	0.327817	60781	61180	495-548	1.000000	83.7
ycf4-cemA_p2	ycf4-cemA	2	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	20	20	50.0	55.0	58.1	57.8	8.4	0.0	0.363893	60781	61180	497-550	1.000000	83.4
ycf4-cemA_p3	ycf4-cemA	3	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	57.8	57.8	0.0	0.0	0.399458	60781	61180	524-577	1.000000	82.8
ycf4-cemA_p4	ycf4-cemA	4	TTTCTTGCGCGTACCAATTG	AGAGAAAGAGACTCCCCAGG	20	20	45.0	55.0	57.7	57.8	8.4	0.0	0.570031	60781	61180	500-553	1.000000	81.7
ycf4-cemA_p5	ycf4-cemA	5	CCTATTTCTTGCGCGTACCA	AGAGAAAGAGACTCCCCAGG	20	20	50.0	55.0	58.4	57.8	8.4	0.0	0.598745	60781	61180	504-557	1.000000	81.4
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	20	22	50.0	45.5	57.9	58.0	0.0	0.0	2.056243	63061	64044	1186-1192	1.000000	68.0
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ACGGTTCATATTCTGGATTGGG	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.231322	63061	64044	1121-1127	1.000000	66.8
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTCGC	CGGTTCATATTCTGGATTGGGT	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.231322	63061	64044	1120-1126	1.000000	66.8
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTCGC	TTCCTCTTTGGCTGATAGGT	20	20	50.0	45.0	57.9	55.8	0.0	0.0	2.229737	63061	64044	1187-1193	1.000000	66.6
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	20	22	50.0	50.0	57.9	57.9	0.0	0.0	2.117200	63061	64044	1185-1191	0.923077	64.3
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	64814	66040	1382-1402	0.923077	76.3
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	64814	66040	1380-1400	0.923077	76.3
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	64814	66040	1371-1391	0.923077	76.1
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	64814	66040	1369-1389	0.923077	76.0
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	64814	66040	1373-1393	0.923077	74.6
rpl33-rps18_p1	rpl33-rps18	1	CAAGCTAAGTCAGGCCTCAG	TGACTGGGCTTGAAATTGCT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.021707	68002	68272	1056-1099	1.000000	86.8
rpl33-rps18_p2	rpl33-rps18	2	TCGCAAGCATACGACTCATG	CGAAAAGTTTGCTTGGATTTACG	20	23	50.0	39.1	58.4	57.5	0.0	0.0	3.920450	68002	68272	346-418	0.846154	50.9
rpl33-rps18_p3	rpl33-rps18	3	TGTCGTTATTGTCGCAAGCA	CGAAAAGTTTGCTTGGATTTACG	20	23	45.0	39.1	58.5	57.5	0.0	0.0	3.976987	68002	68272	357-429	0.846154	50.3
rpl33-rps18_p4	rpl33-rps18	4	TTGTCGTTATTGTCGCAAGC	CGAAAAGTTTGCTTGGATTTACG	20	23	45.0	39.1	57.4	57.5	0.0	0.0	4.067893	68002	68272	358-430	0.846154	49.6
rpl33-rps18_p5	rpl33-rps18	5	CGCAAGCATACGACTCATGA	CGAAAAGTTTGCTTGGATTTACG	20	23	50.0	39.1	58.4	57.5	0.0	0.0	3.920450	68002	68272	386-417	0.769231	46.0
petD_p1	petD	1	TCCGGTAATAGGATCGCCTT	GGAAGAGGGCACTAGTTTCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.016910	75525	76756	2123-2143	1.000000	82.8
petD_p2	petD	2	TGGGTGTATTGACTGCATCG	GGAAGAGGGCACTAGTTTCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.034305	75525	76756	2226-2246	1.000000	82.6
petD_p3	petD	3	GGGTGTATTGACTGCATCGT	GGAAGAGGGCACTAGTTTCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.035716	75525	76756	2225-2245	1.000000	82.6
petD_p4	petD	4	CAGTTCATCGATGGTCAGCA	GGAAGAGGGCACTAGTTTCG	20	20	50.0	55.0	58.0	58.0	24.6	0.0	0.025875	75525	76756	2346-2366	1.000000	82.5
petD_p5	petD	5	TCCGGTAATAGGATCGCCTT	TGCTGTATCCATGCCTGTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048056	75525	76756	2303-2323	1.000000	82.5
rps15-ndhF_p1	rps15-ndhF	1	GTTTGCTGGCTTATTTGGCA	AAATATGTAGGGGGAGGGCG	20	20	45.0	55.0	57.5	58.9	0.0	0.0	1.389159	104774	105218	584-637	1.000000	77.5
rps15-ndhF_p2	rps15-ndhF	2	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATAT	20	19	45.0	57.9	57.5	57.9	0.0	0.0	1.538175	104774	105218	578-631	1.000000	76.3
rps15-ndhF_p3	rps15-ndhF	3	GTTTGCTGGCTTATTTGGCA	GTAGGGGGAGGGCGAATATC	20	20	45.0	60.0	57.5	59.1	0.0	0.0	1.550747	104774	105218	578-631	1.000000	76.2
rps15-ndhF_p4	rps15-ndhF	4	CGTTTGCTGGCTTATTTGGC	AAATATGTAGGGGGAGGGCG	20	20	50.0	55.0	58.7	58.9	0.0	0.0	1.585941	104774	105218	585-638	1.000000	75.9
rps15-ndhF_p5	rps15-ndhF	5	ACGTTTGCTGGCTTATTTGG	AAATATGTAGGGGGAGGGCG	20	20	45.0	55.0	57.3	58.9	0.0	0.0	1.658299	104774	105218	586-639	1.000000	75.3
ndhF_p1	ndhF	1	AAAATCGCGGAAAACCGAAC	ACGAAACATGAGAGCTGCTT	20	20	45.0	45.0	58.0	57.8	6.1	2.5	0.216592	105219	107438	2690-2753	1.000000	82.0
ndhF_p2	ndhF	2	ACCCATCAATGCATGGTGTT	ACGAAACATGAGAGCTGCTT	20	20	45.0	45.0	58.1	57.8	0.0	2.5	0.234500	105219	107438	2791-2854	1.000000	81.8
ndhF_p3	ndhF	3	AATCGCGGAAAACCGAACTA	ACGAAACATGAGAGCTGCTT	20	20	45.0	45.0	57.9	57.8	6.1	2.5	0.259044	105219	107438	2688-2751	1.000000	81.7
ndhF_p4	ndhF	4	TCGCGGAAAACCGAACTATT	ACGAAACATGAGAGCTGCTT	20	20	45.0	45.0	57.9	57.8	6.1	2.5	0.259044	105219	107438	2686-2749	1.000000	81.7
ndhF_p5	ndhF	5	AAAATCGCGGAAAACCGAAC	CTACGGCCTATCCCCCATAT	20	20	45.0	55.0	58.0	57.8	6.1	0.0	0.216962	105219	107438	2936-2999	0.846154	74.4
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	20	24	55.0	33.3	58.1	57.3	0.0	0.0	4.844538	107439	108351	981-1042	1.000000	49.9
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107439	108351	953-1014	1.000000	49.7
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107439	108351	954-1015	1.000000	49.7
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107439	108351	955-1016	1.000000	49.7
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107439	108351	956-1017	1.000000	49.7