Hotspot

rps12

IRb · 107763-108563 · 801 bp

Back to Amorphophallus

Pi 0.0484

MarkerSeek score 80.70

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: IRb
length_bp: 801
variable_sites: 364
pi: 0.0484
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	TGACATGAGCGTGAAAGAGG	ATTGAACGAGGAGCCGTATG	50.0	50.0	57.9	57.8	940-955 bp	0.954545	87.7
rps12_p2	GACATGAGCGTGAAAGAGGT	ATTGAACGAGGAGCCGTATG	50.0	50.0	57.9	57.8	939-954 bp	0.954545	87.7
rps12_p3	CAGTACCTCGACGTGACATG	ATTGAACGAGGAGCCGTATG	55.0	50.0	58.1	57.8	953-968 bp	0.954545	87.6
rps12_p4	TGACATGAGCGTGAAAGAGG	AAGAATTGAACGAGGAGCCG	50.0	50.0	57.9	58.3	944-959 bp	0.954545	87.3
rps12_p5	GACATGAGCGTGAAAGAGGT	AAGAATTGAACGAGGAGCCG	50.0	50.0	57.9	58.3	943-958 bp	0.954545	87.3