Hotspot

accD

LSC · 64855-66084 · 1230 bp

Back to Amorphophallus

Pi 0.11

MarkerSeek score 81.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1230
variable_sites: 392
pi: 0.11
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAGCCAAGGGAGGGTATCTT	TCCCATACATCGCAGCTAGA	50.0	50.0	58.0	58.0	2533-2869 bp	0.590909	75.5
accD_p2	GTCGCGTTCCTATGGAAACT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2394-2979 bp	0.590909	75.4
accD_p3	AAGCCAAGGGAGGGTATCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2477-2813 bp	0.590909	75.3
accD_p4	CATAGCTCAAGCCAAGGGAG	TCCCATACATCGCAGCTAGA	55.0	50.0	58.0	58.0	2541-2877 bp	0.590909	75.3
accD_p5	GTCGCGTTCCTATGGAAACT	TCCCATACATCGCAGCTAGA	50.0	50.0	58.0	58.0	2450-2815 bp	0.545455	73.7