Hotspot

ndhB-trnL-CAA

IRa · 164119-165260 · 1142 bp

Back to Amorphophallus

Pi 0.0614

MarkerSeek score 91.30

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 1142
variable_sites: 390
pi: 0.0614
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 91.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	464-2578 bp	0.954545	89.7
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	462-2576 bp	0.954545	89.7
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	459-2573 bp	0.954545	88.2
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	463-2577 bp	0.954545	87.5
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	461-2575 bp	0.954545	87.4