Hotspot

rps15-ycf1

IRa · 142038-143752 · 1715 bp

Back to Amorphophallus

Pi 0.217

MarkerSeek score 70.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: IRa
length_bp: 1715
variable_sites: 856
pi: 0.217
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.48
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	TCGAAGAAGAGAGATGCGTC	50.0	50.0	58.0	57.2	887-2978 bp	0.636364	70.7
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	CGAAGAAGAGAGATGCGTCT	50.0	50.0	58.0	57.2	886-2977 bp	0.636364	70.6
rps15-ycf1_p3	GCTAGATAATCCCAACGCGT	TCGAAGAAGAGAGATGCGTC	50.0	50.0	57.8	57.2	896-2987 bp	0.636364	69.6
rps15-ycf1_p4	GCTAGATAATCCCAACGCGT	CGAAGAAGAGAGATGCGTCT	50.0	50.0	57.8	57.2	895-2986 bp	0.636364	69.5
rps15-ycf1_p5	TCAGTCGAAGAACACCATGC	TCGAAGAAGAGAGATGCGTC	50.0	50.0	58.2	57.2	760-2851 bp	0.636364	69.1