Hotspot

ndhG-ndhI

SSC · 136757-137625 · 869 bp

Back to Amorphophallus

Pi 0.164

MarkerSeek score 77.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 869
variable_sites: 464
pi: 0.164
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAACTACTCCTAGGCCTCCT	TCATTCAAGGACAGTTACAA	50.0	35.0	56.7	52.0	292-1529 bp	0.954545	50.2
ndhG-ndhI_p2	AAACTACTCCTAGGCCTCCT	TCATTCAAGGACAGTTACAAA	50.0	33.3	56.7	53.0	292-1529 bp	0.954545	50.2
ndhG-ndhI_p3	AAAACTACTCCTAGGCCTCCT	TCATTCAAGGACAGTTACAA	47.6	35.0	57.5	52.0	293-1530 bp	0.954545	50.2
ndhG-ndhI_p4	AAAACTACTCCTAGGCCTCCT	TCATTCAAGGACAGTTACAAA	47.6	33.3	57.5	53.0	293-1530 bp	0.954545	50.2
ndhG-ndhI_p5	AAACTACTCCTAGGCCTCCT	TCATTCAAGGACAGTTACAAAT	50.0	31.8	56.7	53.6	292-1529 bp	0.954545	50.2