Hotspot

trnL-CAA-ndhB

IRb · 103560-104701 · 1142 bp

Back to Amorphophallus

Pi 0.055

MarkerSeek score 90.40

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 1142
variable_sites: 408
pi: 0.055
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 90.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	464-2578 bp	0.909091	87.8
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	462-2576 bp	0.909091	87.8
trnL-CAA-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	459-2573 bp	0.909091	86.3
trnL-CAA-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	463-2577 bp	0.909091	85.7
trnL-CAA-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	461-2575 bp	0.909091	85.5