Hotspot

trnE-UUC-trnT-GGU

LSC · 34987-35893 · 907 bp

Back to Amorphophallus

Pi 0.075
MarkerSeek score 79.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
907
variable_sites
262
pi
0.075
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA TCTACCACTGAGTTAAAAGGGC 50.0 45.5 57.9 57.7 270-1608 bp 0.954545 70.8
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA TCTACCACTGAGTTAAAAGGGC 50.0 45.5 57.9 57.7 268-1606 bp 0.954545 70.8
trnE-UUC-trnT-GGU_p3 GATGTCCTGAACCGCTAGAC TCTACCACTGAGTTAAAAGGGC 55.0 45.5 57.8 57.7 267-1605 bp 0.954545 70.2
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 58.3 57.7 286-1624 bp 0.954545 69.8
trnE-UUC-trnT-GGU_p5 GAGATGTCCTGAACCGCTAG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 57.5 57.7 269-1607 bp 0.954545 67.9