Hotspot

trnH-GUG-psbA

LSC · 70-744 · 675 bp

Back to Amorphophallus

Pi 0.155

MarkerSeek score 77.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 305
pi: 0.155
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CCACTTGGCTACATCCGC	AGCTGCTGTTGAAGTTCCAT	61.1	45.0	58.2	57.7	358-952 bp	0.909091	68.6
trnH-GUG-psbA_p2	CCACTTGGCTACATCCGC	GCTGCTGTTGAAGTTCCATC	61.1	50.0	58.2	57.4	357-951 bp	0.909091	65.9
trnH-GUG-psbA_p3	CCACTTGGCTACATCCGC	TAGCTGCTGTTGAAGTTCCA	61.1	45.0	58.2	57.1	359-953 bp	0.909091	63.4
trnH-GUG-psbA_p4	CCACTTGGCTACATCCGC	TTAGCTGCTGTTGAAGTTCCA	61.1	42.9	58.2	57.8	360-954 bp	0.909091	61.2
trnH-GUG-psbA_p5	CCACTTGGCTACATCCGC	TAGCTGCTGTTGAAGTTCCAT	61.1	42.9	58.2	57.6	359-953 bp	0.909091	59.5