Hotspot

psaC

SSC · 120740-120985 · 246 bp

Back to Amorpha

Pi 0

MarkerSeek score 23.50

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TTTTGAGCACGGGTTTTTGC	TCGTTGATTCATCTGGTGTCT	45.0	42.9	58.4	57.0	401-422 bp	1.0	61.5
psaC_p2	TTTTGAGCACGGGTTTTTGC	ATCGTTGATTCATCTGGTGTCT	45.0	40.9	58.4	57.5	402-423 bp	1.0	57.3
psaC_p3	CACGGGTTTTTGCCAGTAAA	TCGTTGATTCATCTGGTGTCT	45.0	42.9	57.1	57.0	394-415 bp	1.0	57.3
psaC_p4	TTTTGAGCACGGGTTTTTGC	TCGTTGATTCATCTGGTGTC	45.0	45.0	58.4	55.5	401-422 bp	1.0	57.1
psaC_p5	TTTTGAGCACGGGTTTTTGC	CGTTGATTCATCTGGTGTCT	45.0	45.0	58.4	55.5	400-421 bp	1.0	57.0