Hotspot

ndhD-psaC

SSC · 120651-120739 · 89 bp

Back to Amorpha

Pi 0.0375

MarkerSeek score 63.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 89
variable_sites: 5
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ATTAAGGAACCCCCGGCTAT	AAACAACTCGAAGCATGGGT	50.0	45.0	58.2	58.0	213-214 bp	1.0	80.4
ndhD-psaC_p2	ATTAAGGAACCCCCGGCTAT	GGCATGAAACAACTCGAAGC	50.0	50.0	58.2	58.0	219-220 bp	1.0	80.2
ndhD-psaC_p3	CCCGGCTATTGGCAAAACTA	AAACAACTCGAAGCATGGGT	50.0	45.0	58.2	58.0	202-203 bp	1.0	79.9
ndhD-psaC_p4	CCCGGCTATTGGCAAAACTA	GGCATGAAACAACTCGAAGC	50.0	50.0	58.2	58.0	208-209 bp	1.0	79.7
ndhD-psaC_p5	GAAGATTAAGGAACCCCCGG	AAACAACTCGAAGCATGGGT	55.0	45.0	57.7	58.0	217-218 bp	1.0	78.9