Hotspot

rps3-rps19

LSC · 87249-87830 · 582 bp

Back to Amorpha

Pi 0.00239

MarkerSeek score 63.80

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 2
pi: 0.00239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	TAAACTTCCGAGGACATGCG	50.0	50.0	58.4	58.0	641-666 bp	1.0	80.0
rps3-rps19_p2	CAAGTCTGAAACCAAGCGGA	TCCGAGGACATGCGAAAAAT	50.0	45.0	58.4	57.9	635-660 bp	1.0	79.2
rps3-rps19_p3	CAAGTCTGAAACCAAGCGGA	TTCCGAGGACATGCGAAAAA	50.0	45.0	58.4	58.1	636-661 bp	1.0	79.2
rps3-rps19_p4	AGTCTGAAACCAAGCGGATT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	639-664 bp	1.0	78.8
rps3-rps19_p5	AAGTCTGAAACCAAGCGGAT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	640-665 bp	1.0	78.7