Hotspot

petN-psbM

LSC · 74794-76130 · 1337 bp

Back to Amorpha

Pi 0.00449

MarkerSeek score 61.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1337
variable_sites: 9
pi: 0.00449
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCCCTGTTCATTC	45.0	50.0	58.0	57.9	1475-1492 bp	1.0	80.9
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCCCTGTTCATTC	50.0	50.0	57.8	57.9	1481-1498 bp	1.0	79.4
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	GCTACTGCCCTGTTCATTCT	45.0	50.0	58.0	57.6	1474-1491 bp	1.0	78.5
petN-psbM_p4	CCCTCGTAGTATGGGGAAGA	TGCTACTGCCCTGTTCATTC	55.0	50.0	57.6	57.9	1431-1448 bp	1.0	78.0
petN-psbM_p5	TCCCTCGTAGTATGGGGAAG	TGCTACTGCCCTGTTCATTC	55.0	50.0	57.6	57.9	1432-1449 bp	1.0	78.0