Hotspot

rpoB-trnC-GCA

LSC · 72717-73813 · 1097 bp

Back to Amorpha

Pi 0.00304

MarkerSeek score 61.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1097
variable_sites: 4
pi: 0.00304
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTCCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.2	58.1	1186-1190 bp	1.0	78.3
rpoB-trnC-GCA_p2	TAAGCCCCGATCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1252-1256 bp	1.0	77.2
rpoB-trnC-GCA_p3	CATTCCCTCATTTCCACCCC	CTTATCGATCAGGCGACACC	55.0	55.0	58.2	58.2	1205-1209 bp	1.0	77.1
rpoB-trnC-GCA_p4	GCCCCGATCAATGAACCTAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.4	58.1	1249-1253 bp	1.0	76.7
rpoB-trnC-GCA_p5	GTTCTTCTGTTAAGCCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1262-1266 bp	1.0	76.2