Hotspot

psaA-ycf3

LSC · 43504-44322 · 819 bp

Back to Amorpha

Pi 0.00326

MarkerSeek score 61.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 4
pi: 0.00326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AATGACCAGGTTTTGCCCAT	AGCCCTTACCCCTGGTAATT	45.0	50.0	58.0	58.0	978-980 bp	1.0	80.2
psaA-ycf3_p2	ATGACCAGGTTTTGCCCATT	AGCCCTTACCCCTGGTAATT	45.0	50.0	58.0	58.0	977-979 bp	1.0	80.2
psaA-ycf3_p3	AATGACCAGGTTTTGCCCAT	AGCTATAGCCCTTACCCCTG	45.0	55.0	58.0	58.0	984-986 bp	1.0	80.0
psaA-ycf3_p4	ATGACCAGGTTTTGCCCATT	AGCTATAGCCCTTACCCCTG	45.0	55.0	58.0	58.0	983-985 bp	1.0	80.0
psaA-ycf3_p5	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	965-967 bp	1.0	79.6