Hotspot

psbE-petL

LSC · 21671-23122 · 1452 bp

Back to Amorpha

Pi 0.00413

MarkerSeek score 61.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1452
variable_sites: 9
pi: 0.00413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCCACTAGTAGAAAACCGA	45.0	45.0	58.0	55.9	1606-1609 bp	1.0	63.7
psbE-petL_p2	TGACAAATAACCAACCCGCA	GCTGAGGTTGTAGTTAAAGCC	45.0	47.6	58.0	56.9	1623-1626 bp	1.0	63.6
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.9	55.9	1595-1598 bp	1.0	63.4
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCTGAGGTTGTAGTTAAAGCC	50.0	47.6	57.9	56.9	1612-1615 bp	1.0	63.3
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.8	55.9	1518-1521 bp	1.0	62.6