Hotspot

ycf1

IRa · 132384-137840 · 5457 bp

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Pi 0.0173
MarkerSeek score 55.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRa
length_bp
5457
variable_sites
176
pi
0.0173
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCGGTAATTGGGCCAAAGAA ACATATCGGTGCCAAACTTGA 45.0 42.9 57.4 57.9 1156-1189 bp 1.0 76.4
ycf1_p2 TGGTGCTTACTCTTTTCGCT ACATATCGGTGCCAAACTTGA 45.0 42.9 57.5 57.9 856-858 bp 0.75 62.1
ycf1_p3 TGGTGCTTACTCTTTTCGCT TCGGTGCCAAACTTGAAAAA 45.0 40.0 57.5 56.7 851-853 bp 0.75 60.0
ycf1_p4 TTCCACCCGTTACAAAAGCT ACATATCGGTGCCAAACTTGA 45.0 42.9 57.6 57.9 797 bp 0.5 49.6
ycf1_p5 TTCCACCCGTTACAAAAGCT TCGGTGCCAAACTTGAAAAA 45.0 40.0 57.6 56.7 792 bp 0.5 47.5