Hotspot

rpl2-trnH-GUG

IRa · 163071-163113 · 43 bp

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Pi 0.105
MarkerSeek score 73.20
Primer available yes
Label rpl2-trnH-GUG

Feature Metadata

label_name
rpl2-trnH-GUG
feature_type
igs
region
IRa
length_bp
43
variable_sites
7
pi
0.105
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-trnH-GUG_p1 GTTATCCTGCGCTTGGAAGA TCTCTTCCCTCGTGTTGAGT 50.0 50.0 58.0 58.0 427-439 bp 1.0 84.6
rpl2-trnH-GUG_p2 GGGTAGAGCCGGATCTAAGT TCTCTTCCCTCGTGTTGAGT 55.0 50.0 58.0 58.0 564-576 bp 1.0 83.7
rpl2-trnH-GUG_p3 TGGGTAGAGCCGGATCTAAG TCTCTTCCCTCGTGTTGAGT 55.0 50.0 58.0 58.0 565-577 bp 1.0 83.7
rpl2-trnH-GUG_p4 GAGCCGGATCTAAGTGTTGG TCTCTTCCCTCGTGTTGAGT 55.0 50.0 58.1 58.0 559-571 bp 1.0 83.3
rpl2-trnH-GUG_p5 GGGTAGAGCCGGATCTAAGT TGCCCGAGACCAAGTTACTA 55.0 50.0 58.0 58.1 593-605 bp 1.0 83.0