Hotspot

ndhF-rpl32

SSC · 120708-121330 · 623 bp

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Pi 0.0322
MarkerSeek score 72.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
623
variable_sites
36
pi
0.0322
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCGGTCGTGTGAACCAAAAT TTGAACGTCCGGTAGAAACC 45.0 50.0 58.0 57.8 1232-1267 bp 1.0 83.8
ndhF-rpl32_p2 TACATGAAGGGGGAATTGCG TTGAACGTCCGGTAGAAACC 50.0 50.0 57.9 57.8 1468-1503 bp 1.0 83.0
ndhF-rpl32_p3 GCGCAGATTTAGCAATTGCA TTGAACGTCCGGTAGAAACC 45.0 50.0 58.1 57.8 1451-1486 bp 1.0 82.8
ndhF-rpl32_p4 TGCGCAGATTTAGCAATTGC TTGAACGTCCGGTAGAAACC 45.0 50.0 58.1 57.8 1452-1487 bp 1.0 82.8
ndhF-rpl32_p5 GGGAATTGCGCAGATTTAGC TTGAACGTCCGGTAGAAACC 50.0 50.0 57.9 57.8 1458-1493 bp 1.0 82.5