Hotspot

ycf1-ndhF

SSC · 118429-118496 · 68 bp

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Pi 0.0613
MarkerSeek score 72.10
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
68
variable_sites
6
pi
0.0613
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.85
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 CGCGGTACCCATCCTTTTAT GGGGTTGTTGTTGCAAGTTT 50.0 45.0 57.8 57.6 494-589 bp 1.0 80.6
ycf1-ndhF_p2 CGCGGTACCCATCCTTTTAT ATGGGAGGTGGACGAATTTC 50.0 50.0 57.8 57.6 449-544 bp 1.0 80.5
ycf1-ndhF_p3 CAATATCGCGGTACCCATCC GGGGTTGTTGTTGCAAGTTT 55.0 45.0 58.3 57.6 500-595 bp 1.0 80.3
ycf1-ndhF_p4 TCGAAGGTCAATGCACAACT GGGGTTGTTGTTGCAAGTTT 45.0 45.0 57.7 57.6 761-856 bp 1.0 80.1
ycf1-ndhF_p5 TCGAAGGTCAATGCACAACT ATGGGAGGTGGACGAATTTC 45.0 50.0 57.7 57.6 716-811 bp 1.0 79.9