Hotspot

trnH-GUG-rpl2

IRb · 89263-89305 · 43 bp

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Pi 0.0736
MarkerSeek score 73.20
Primer available yes
Label trnH-GUG-rpl2

Feature Metadata

label_name
trnH-GUG-rpl2
feature_type
igs
region
IRb
length_bp
43
variable_sites
6
pi
0.0736
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rpl2_p1 TCTCTTCCCTCGTGTTGAGT GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 438-439 bp 1.0 81.8
trnH-GUG-rpl2_p2 TCTCTTCCCTCGTGTTGAGT GGGTAGAGCCGGATCTAAGT 50.0 55.0 58.0 58.0 575-576 bp 1.0 81.6
trnH-GUG-rpl2_p3 TCTCTTCCCTCGTGTTGAGT TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.0 58.0 576-577 bp 1.0 81.6
trnH-GUG-rpl2_p4 TCTCTTCCCTCGTGTTGAGT GAGCCGGATCTAAGTGTTGG 50.0 55.0 58.0 58.1 570-571 bp 1.0 81.2
trnH-GUG-rpl2_p5 TGCCCGAGACCAAGTTACTA GGGTAGAGCCGGATCTAAGT 50.0 55.0 58.1 58.0 604-605 bp 1.0 81.0