Hotspot

rpl22-rps19

IRb · 88568-88747 · 180 bp

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Pi 0.0773
MarkerSeek score 74.70
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
180
variable_sites
23
pi
0.0773
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 ATGTGACAAGTGGGCCTTTT ACTCAACACGAGGGAAGAGA 45.0 50.0 57.9 58.0 651-839 bp 1.0 86.4
rpl22-rps19_p2 CCGTGCAATTCCTTCGAAAC ACTCAACACGAGGGAAGAGA 50.0 50.0 58.0 58.0 965-1153 bp 1.0 86.1
rpl22-rps19_p3 CGTGCAATTCCTTCGAAACC ACTCAACACGAGGGAAGAGA 50.0 50.0 58.0 58.0 964-1152 bp 1.0 86.1
rpl22-rps19_p4 CCGTGCAATTCCTTCGAAAC GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1383-1571 bp 1.0 85.8
rpl22-rps19_p5 CGTGCAATTCCTTCGAAACC GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1382-1570 bp 1.0 85.8