Hotspot

rps11-rpl36

LSC · 84037-84158 · 122 bp

Back to Amomum

Pi 0.0441
MarkerSeek score 71.40
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
122
variable_sites
9
pi
0.0441
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 GAAACCACTCGACCTCGTAC TCCGGGTCCGCTTAGATAAT 55.0 50.0 58.0 58.0 671-675 bp 1.0 82.4
rps11-rpl36_p2 TCATAACTCCTGCTCGTTGC TCCGGGTCCGCTTAGATAAT 50.0 50.0 58.0 58.0 799-803 bp 1.0 82.3
rps11-rpl36_p3 TCATAACTCCTGCTCGTTGC GAAGGATCCGCCGTAGTTTT 50.0 50.0 58.0 58.0 751-755 bp 1.0 82.3
rps11-rpl36_p4 GTAATGCTGCGTCTCTTCCA TCCGGGTCCGCTTAGATAAT 50.0 50.0 58.0 58.0 835-839 bp 1.0 82.2
rps11-rpl36_p5 GTAATGCTGCGTCTCTTCCA GAAGGATCCGCCGTAGTTTT 50.0 50.0 58.0 58.0 787-791 bp 1.0 82.2