Hotspot

atpH-atpI

LSC · 14427-15820 · 1394 bp

Pi 0.036

MarkerSeek score 73.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1394
variable_sites: 66
pi: 0.036
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.0	57.9	976-1566 bp	1.0	87.0
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.1	57.9	891-1481 bp	1.0	86.8
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.9	57.9	949-1539 bp	1.0	86.5
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	ATTTTTGCAACCTTAGCCGC	55.0	45.0	58.2	57.9	953-1543 bp	1.0	85.9
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.8	57.9	908-1498 bp	1.0	85.8