Hotspot

ycf2

IRb · 86318-93343 · 7026 bp

Back to Ammopiptanthus

Pi 0.00201

MarkerSeek score 54.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 7026
variable_sites: 14
pi: 0.00201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCCTTTTCGCTCCGCTTAG	CAGCTCCGTATCAAGGTCAC	57.9	55.0	58.6	58.1	213-276 bp	1.0	70.4
ycf2_p2	CTGGACGATCCTATTTGGTCA	CAGCTCCGTATCAAGGTCAC	47.6	55.0	57.2	58.1	149-212 bp	1.0	70.2
ycf2_p3	GGGAAACCCTTTTCGCTCC	CAGCTCCGTATCAAGGTCAC	57.9	55.0	58.7	58.1	219-282 bp	1.0	69.4
ycf2_p4	CCCTTTTCGCTCCGCTTAG	CAAGGTCACGGTCGATATCG	57.9	55.0	58.6	58.2	202-265 bp	1.0	69.2
ycf2_p5	CCCTTTTCGCTCCGCTTAG	GTCACGGTCGATATCGTCAC	57.9	55.0	58.6	58.3	198-261 bp	1.0	69.2