Hotspot

rps19-rpl2

IRb · 83832-83906 · 75 bp

Back to Ammopiptanthus

Pi 0.0137

MarkerSeek score 62.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 75
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGTTCTTGACCAAGT	GGGCCCCAATTGGTAGAAAA	40.9	50.0	57.0	58.1	310-312 bp	1.0	56.5
rps19-rpl2_p2	TGGTAGATGTTCTTGACCAAGT	TTGATTCTTCGTCGCCGTAG	40.9	50.0	57.0	58.1	218-220 bp	1.0	56.5
rps19-rpl2_p3	TGGTAGATGTTCTTGACCAAGT	TTGGGGTTATCCTGCACTTG	40.9	50.0	57.0	57.8	276-278 bp	1.0	55.4
rps19-rpl2_p4	TGGTAGATGTTCTTGACCAAGT	GCAACTCCTTGGGGTTATCC	40.9	55.0	57.0	58.2	284-286 bp	1.0	55.2
rps19-rpl2_p5	TCTTTTGCTGCTTTTGTGTT	GGGCCCCAATTGGTAGAAAA	35.0	50.0	54.8	58.1	278-280 bp	1.0	54.9