Hotspot

rpl16-rps3

LSC · 81066-82337 · 1272 bp

Back to Ammopiptanthus

Pi 0.00158

MarkerSeek score 60.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1272
variable_sites: 2
pi: 0.00158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CTCAAGTGCCTGAAGAGCAT	TCCTCTACAAACCATTCGAGC	50.0	47.6	57.9	57.8	1444-1449 bp	1.0	69.5
rpl16-rps3_p2	CTCAAGTGCCTGAAGAGCAT	CCTCTACAAACCATTCGAGCT	50.0	47.6	57.9	57.7	1443-1448 bp	1.0	69.4
rpl16-rps3_p3	CTAGATGTGATCCAAGCGGG	TCCTCTACAAACCATTCGAGC	55.0	47.6	57.8	57.8	1464-1469 bp	1.0	69.1
rpl16-rps3_p4	CTAGATGTGATCCAAGCGGG	CCTCTACAAACCATTCGAGCT	55.0	47.6	57.8	57.7	1463-1468 bp	1.0	69.0
rpl16-rps3_p5	CTCAAGTGCCTGAAGAGCAT	GGTAGGGTTCCTCTACAAACC	50.0	52.4	57.9	57.4	1452-1457 bp	1.0	66.5