Hotspot

rps11-rpl36

LSC · 78622-78953 · 332 bp

Back to Ammopiptanthus

Pi 0.00301

MarkerSeek score 54.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 1
pi: 0.00301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTTTACGCGAACC	AGGAGGGGGCGAATTATAGT	50.0	50.0	58.1	57.6	436 bp	1.0	75.8
rps11-rpl36_p2	TTACGCGAACCAATACGTCC	AGGAGGGGGCGAATTATAGT	50.0	50.0	58.4	57.6	451 bp	1.0	73.9
rps11-rpl36_p3	ACGTCCATTTTTACGCGAAC	AGGAGGGGGCGAATTATAGT	45.0	50.0	57.4	57.6	437 bp	1.0	72.2
rps11-rpl36_p4	CGTCCATTTTTACGCGAACC	AAGGGCCTCTGTTCGTAAAA	50.0	45.0	58.1	57.1	482 bp	1.0	71.6
rps11-rpl36_p5	CGTCCATTTTTACGCGAACC	CGTAGGAGGGGGCGAATTAT	50.0	55.0	58.1	59.0	439 bp	1.0	71.0