Hotspot

psbT-psbN

LSC · 73419-73480 · 62 bp

Back to Ammopiptanthus

Pi 0.0323

MarkerSeek score 67.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 2
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AGAGAACCCCCTAAAGTTCCA	CCATTCGAAGAACATGGGGA	47.6	50.0	58.0	57.6	125 bp	1.0	69.9
psbT-psbN_p2	AGAGAACCCCCTAAAGTTCCA	TCCATTCGAAGAACATGGGG	47.6	50.0	58.0	57.6	126 bp	1.0	69.9
psbT-psbN_p3	AGAGAACCCCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	47.6	50.0	58.0	57.4	151 bp	1.0	68.6
psbT-psbN_p4	GAACCCCCTAAAGTTCCAACT	CCATTCGAAGAACATGGGGA	47.6	50.0	57.5	57.6	122 bp	1.0	65.8
psbT-psbN_p5	GAACCCCCTAAAGTTCCAACT	TCCATTCGAAGAACATGGGG	47.6	50.0	57.5	57.6	123 bp	1.0	65.8