Hotspot

psaJ-rpl33

LSC · 66230-66652 · 423 bp

Back to Ammopiptanthus

Pi 0.0118

MarkerSeek score 61.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 5
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TACTCTATGGTTCGGGGCTT	CGGATATCTTTACCCTTGGCC	50.0	52.4	57.8	58.2	537 bp	1.0	69.6
psaJ-rpl33_p2	GCTTTAGCGGGTCTCTTGAT	CGGATATCTTTACCCTTGGCC	50.0	52.4	57.7	58.2	521 bp	1.0	68.5
psaJ-rpl33_p3	CTATGGTTCGGGGCTTTAGC	CGGATATCTTTACCCTTGGCC	55.0	52.4	58.4	58.2	533 bp	1.0	67.7
psaJ-rpl33_p4	AGTACTCTATGGTTCGGGGC	CGGATATCTTTACCCTTGGCC	55.0	52.4	58.6	58.2	539 bp	1.0	66.3
psaJ-rpl33_p5	GTACTCTATGGTTCGGGGCT	CGGATATCTTTACCCTTGGCC	55.0	52.4	58.6	58.2	538 bp	1.0	66.3