Hotspot

trnC-GCA-rpoB

LSC · 13821-14576 · 756 bp

Back to Ammopiptanthus

Pi 0.004

MarkerSeek score 61.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 3
pi: 0.004
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CTTGTTGAGATCAGGCGACA	CGTCTTTTAAGCCCCGATCA	50.0	50.0	57.9	58.0	936-939 bp	1.0	79.9
trnC-GCA-rpoB_p2	TTGTTGAGATCAGGCGACAC	CGTCTTTTAAGCCCCGATCA	50.0	50.0	58.2	58.0	935-938 bp	1.0	79.0
trnC-GCA-rpoB_p3	CTTGTTGAGATCAGGCGACA	AGTTCGTCTTTTAAGCCCCG	50.0	50.0	57.9	58.2	940-943 bp	1.0	78.5
trnC-GCA-rpoB_p4	CTTGTTGAGATCAGGCGACA	GTTCGTCTTTTAAGCCCCGA	50.0	50.0	57.9	58.2	939-942 bp	1.0	78.5
trnC-GCA-rpoB_p5	GGGGAAAAGGGATTTGCAGT	CGTCTTTTAAGCCCCGATCA	50.0	50.0	58.4	58.0	902-905 bp	1.0	77.8