Hotspot

psaC-ndhE

SSC · 121075-121364 · 290 bp

Back to Ammannia

Pi 0.0415

MarkerSeek score 72.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 290
variable_sites: 17
pi: 0.0415
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGCTCGGACACATTGAGTAC	TCGTAACAGAAAATCAACTCGT	55.0	36.4	58.4	56.1	397-405 bp	1.0	51.4
psaC-ndhE_p2	GCTCGGACACATTGAGTACA	TCGTAACAGAAAATCAACTCGT	50.0	36.4	57.6	56.1	396-404 bp	1.0	51.3
psaC-ndhE_p3	AGGCTCGGACACATTGAGTA	TCGTAACAGAAAATCAACTCGT	50.0	36.4	58.4	56.1	398-406 bp	1.0	50.7
psaC-ndhE_p4	GGCTCGGACACATTGAGTAC	CGTAACAGAAAATCAACTCGT	55.0	38.1	58.4	54.6	396-404 bp	1.0	47.4
psaC-ndhE_p5	GCTCGGACACATTGAGTACA	CGTAACAGAAAATCAACTCGT	50.0	38.1	57.6	54.6	395-403 bp	1.0	47.4