Hotspot

trnL-UAG-ccsA

SSC · 117862-117954 · 93 bp

Back to Ammannia

Pi 0.0932
MarkerSeek score 74.20
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
93
variable_sites
13
pi
0.0932
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT GATCCCCATGCCTCATTAGC 50.0 55.0 58.0 58.2 894-914 bp 1.0 83.6
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG GATCCCCATGCCTCATTAGC 50.0 55.0 58.0 58.2 893-913 bp 1.0 83.6
trnL-UAG-ccsA_p3 GTGAAATCGGTAGACACGCT CTTAATGGGAAATGGGCCGA 50.0 50.0 58.0 57.9 370-372 bp 1.0 83.3
trnL-UAG-ccsA_p4 TGAAATCGGTAGACACGCTG CTTAATGGGAAATGGGCCGA 50.0 50.0 58.0 57.9 369-371 bp 1.0 83.3
trnL-UAG-ccsA_p5 TTTCCCCATCGGTTCACTTG CTTAATGGGAAATGGGCCGA 50.0 50.0 58.1 57.9 656-676 bp 1.0 82.2