Hotspot

rpl36-infA

LSC · 83863-84017 · 155 bp

Back to Ammannia

Pi 0.0516

MarkerSeek score 70.10

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 12
pi: 0.0516
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	ACTTTTACGTGAACCACCCC	GCGGGATGGGGATTGTAATT	50.0	50.0	58.0	58.0	870-876 bp	1.0	82.5
rpl36-infA_p2	CCTTGAATCCACAGGTACCG	GCGGGATGGGGATTGTAATT	55.0	50.0	58.0	58.0	994-1000 bp	1.0	82.3
rpl36-infA_p3	CTTGAATCCACAGGTACCGG	GCGGGATGGGGATTGTAATT	55.0	50.0	58.0	58.0	993-999 bp	1.0	82.3
rpl36-infA_p4	CCTTGAATCCACAGGTACCG	AAAGGGACGGTTCGAATAGC	55.0	50.0	58.0	58.0	1235-1241 bp	1.0	82.2
rpl36-infA_p5	CTTGAATCCACAGGTACCGG	AAAGGGACGGTTCGAATAGC	55.0	50.0	58.0	58.0	1234-1240 bp	1.0	82.2