Hotspot

petD-rpoA

LSC · 81923-82137 · 215 bp

Back to Ammannia

Pi 0.0496

MarkerSeek score 71.30

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 15
pi: 0.0496
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	ACAACCGATTTACCGAACAA	50.0	40.0	58.1	55.6	296-303 bp	1.0	64.8
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	ACAACCGATTTACCGAACAA	50.0	40.0	58.1	55.6	297-304 bp	1.0	64.8
petD-rpoA_p3	TTGATTGGTACCGTAGTGGC	ACAACCGATTTACCGAACAA	50.0	40.0	57.6	55.6	321-328 bp	1.0	62.0
petD-rpoA_p4	GGTTGGGTATTGGAGCAACA	ACAACCGATTTACCGAACAAT	50.0	38.1	58.1	56.2	296-303 bp	1.0	61.3
petD-rpoA_p5	TGGTTGGGTATTGGAGCAAC	ACAACCGATTTACCGAACAAT	50.0	38.1	58.1	56.2	297-304 bp	1.0	61.3