Hotspot

petA-psbJ

LSC · 66645-67531 · 887 bp

Back to Ammannia

Pi 0.0334

MarkerSeek score 66.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 887
variable_sites: 44
pi: 0.0334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TAGGCGGGAATAGAGGAAGG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1675-1689 bp	1.0	82.5
petA-psbJ_p2	TAGGCGGGAATAGAGGAAGG	ACGACACAATCAAACCCGAA	55.0	45.0	58.0	58.0	1678-1692 bp	1.0	82.5
petA-psbJ_p3	TAGGCGGGAATAGAGGAAGG	TGGACTAGCTGTACCTACCG	55.0	55.0	58.0	58.0	1767-1781 bp	1.0	82.3
petA-psbJ_p4	TAGGCGGGAATAGAGGAAGG	GGACTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1766-1780 bp	1.0	82.3
petA-psbJ_p5	TAGGCGGGAATAGAGGAAGG	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1997-2011 bp	1.0	82.0