Hotspot

ndhK-ndhC

LSC · 52978-53032 · 55 bp

Back to Ammannia

Pi 0.0364

MarkerSeek score 72.90

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 3
pi: 0.0364
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TTTGGGTTGTTCGAGCAAGT	ATGGCGAAAGGGAGCATTAG	45.0	50.0	58.2	58.0	127-129 bp	1.0	81.0
ndhK-ndhC_p2	TTTGGGTTGTTCGAGCAAGT	GGCGAAAGGGAGCATTAGAA	45.0	50.0	58.2	58.0	125-127 bp	1.0	80.9
ndhK-ndhC_p3	TTTGGGTTGTTCGAGCAAGT	TTTATGCATGGCGAAAGGGA	45.0	45.0	58.2	57.9	134-136 bp	1.0	79.9
ndhK-ndhC_p4	GGCCACAAACTTGAGAGTCT	ATGGCGAAAGGGAGCATTAG	50.0	50.0	57.7	58.0	191-193 bp	1.0	79.8
ndhK-ndhC_p5	GGCCACAAACTTGAGAGTCT	GGCGAAAGGGAGCATTAGAA	50.0	50.0	57.7	58.0	189-191 bp	1.0	79.7