Hotspot

trnF-GAA-ndhJ

LSC · 50913-51697 · 785 bp

Back to Ammannia

Pi 0.0256

MarkerSeek score 70.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 29
pi: 0.0256
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	927-929 bp	1.0	84.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	891-893 bp	1.0	84.4
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	915-917 bp	1.0	83.6
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	936-938 bp	1.0	82.5
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	CCATCCACGACTGAAACGTA	55.0	50.0	58.0	57.6	961-963 bp	1.0	82.3