Hotspot

petN-psbM

LSC · 30041-30985 · 945 bp

Back to Ammannia

Pi 0.0289

MarkerSeek score 66.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 945
variable_sites: 40
pi: 0.0289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TCATCCCATTGAATCGACGG	45.0	50.0	58.0	57.8	1186-1216 bp	1.0	83.4
petN-psbM_p2	TCCCTTTTTGACTCTGCACC	TCATCCCATTGAATCGACGG	50.0	50.0	58.0	57.8	1289-1319 bp	1.0	82.9
petN-psbM_p3	TGTGCTTAACGTTTTCCCGA	TCATCCCATTGAATCGACGG	45.0	50.0	58.0	57.8	1381-1411 bp	1.0	82.5
petN-psbM_p4	TTAGTGGTTGTGGAAAGGGC	TCATCCCATTGAATCGACGG	50.0	50.0	58.0	57.8	1746-1771 bp	1.0	82.4
petN-psbM_p5	GTTGTGGAAAGGGCTGAAGA	TCATCCCATTGAATCGACGG	50.0	50.0	58.0	57.8	1740-1765 bp	1.0	82.4