Hotspot

atpH-atpI

LSC · 14682-15655 · 974 bp

Back to Ammannia

Pi 0.0295

MarkerSeek score 70.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 974
variable_sites: 41
pi: 0.0295
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	1036-1121 bp	1.0	85.4
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	1092-1177 bp	1.0	84.5
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	1037-1122 bp	1.0	84.3
atpH-atpI_p4	GCGATACCTTCTACAGCCTG	ATAGGGGAATCCATGGAGGG	55.0	55.0	57.9	57.9	1123-1208 bp	1.0	84.1
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	55.0	55.0	58.2	57.9	1096-1181 bp	1.0	83.9