Hotspot

trnR-UCU-atpA

LSC · 10728-11044 · 317 bp

Back to Ammannia

Pi 0.0597

MarkerSeek score 72.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 317
variable_sites: 24
pi: 0.0597
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	CTCTCACGGTGGAAGAACAG	50.0	55.0	58.0	57.9	789-846 bp	1.0	86.8
trnR-UCU-atpA_p2	ACCCTTAGCCTTCCAAGCTA	CTCTCACGGTGGAAGAACAG	50.0	55.0	58.0	57.9	795-852 bp	1.0	86.7
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	CGAAGCTTATCCGGGAGATG	50.0	55.0	58.0	57.9	1220-1277 bp	1.0	85.7
trnR-UCU-atpA_p4	ACCCTTAGCCTTCCAAGCTA	CGAAGCTTATCCGGGAGATG	50.0	55.0	58.0	57.9	1226-1283 bp	1.0	85.7
trnR-UCU-atpA_p5	AGCCTTCCAAGCTAACGATG	ATCCCTCCAAACAAGCACAG	50.0	50.0	58.0	58.1	1284-1341 bp	1.0	85.5