Hotspot

trnG-UCC-trnR-UCU

LSC · 10485-10655 · 171 bp

Back to Ammannia

Pi 0.0394
MarkerSeek score 72.90
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
171
variable_sites
10
pi
0.0394
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 251-270 bp 1.0 68.9
trnG-UCC-trnR-UCU_p2 ACCCTTAGCCTTCCAAGCTA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 257-276 bp 1.0 68.8
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 257-276 bp 1.0 68.1
trnG-UCC-trnR-UCU_p4 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 263-282 bp 1.0 68.0
trnG-UCC-trnR-UCU_p5 CTAACGATGCGGGTTCGATT AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.4 56.9 240-259 bp 1.0 66.0