Hotspot

trnQ-UUG-psbK

LSC · 7637-8028 · 392 bp

Back to Ammannia

Pi 0.0341

MarkerSeek score 70.30

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 18
pi: 0.0341
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	AGGTATTTCCTGGGACGGAA	ACTACTCGAATAAAGGGCGG	50.0	50.0	58.0	57.1	522-533 bp	1.0	77.3
trnQ-UUG-psbK_p2	AGGTATTTCCTGGGACGGAA	ACTCGAATAAAGGGCGGAAT	50.0	45.0	58.0	57.0	519-530 bp	1.0	76.4
trnQ-UUG-psbK_p3	TATTTCCTGGGACGGAAGGA	ACTACTCGAATAAAGGGCGG	50.0	50.0	57.7	57.1	519-530 bp	1.0	75.6
trnQ-UUG-psbK_p4	AGGTATTTCCTGGGACGGAA	CTACTCGAATAAAGGGCGGA	50.0	50.0	58.0	56.8	521-532 bp	1.0	75.0
trnQ-UUG-psbK_p5	GTATTTCCTGGGACGGAAGG	ACTACTCGAATAAAGGGCGG	55.0	50.0	57.7	57.1	520-531 bp	1.0	75.0