Hotspot

rps16-trnQ-UUG

LSC · 6198-7564 · 1367 bp

Back to Ammannia

Pi 0.0355
MarkerSeek score 72.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1367
variable_sites
60
pi
0.0355
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 974-1435 bp 1.0 85.6
rps16-trnQ-UUG_p2 TCATGTCTGTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 990-1451 bp 1.0 85.4
rps16-trnQ-UUG_p3 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 978-1439 bp 1.0 84.1
rps16-trnQ-UUG_p4 CAAGTCGCACGTTGCTTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.5 58.1 980-1441 bp 1.0 83.5
rps16-trnQ-UUG_p5 GTCGCACGTTGCTTTTTACC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 977-1438 bp 1.0 82.3